Cloning and Expression Vectors for Gene Function Analysis (Biofocus (Westborough, Mass.).)

Reprints 43 articles from the journal BioTechnique in sections on cloning vectors and strategies, protein expression and purification, gene tagging and epitope tagging strategies, reporter gene technologies, special purpose vectors, and in-vitro protein expression technologies. Among specific topics high-throughput transformation and plating using petristrips, the in-vivo labeling of over-expressed recombinant proteins in E. coli , the multiple epitope tagging of expressed proteins for enhanced detection, applications of green fluorescent proteins in plants, highly efficient eukaryotic gene expression vectors for peptide secretion, and the cell-free synthesis and affinity isolation of proteins on a nanomole scale. Updates have been added to some of the articles. Annotation c. Book News, Inc., Portland, OR (booknews.com)