The PCR Technique : DNA Sequencing

Reference

Ellingboe, James

Catalogs & Directories

Eaton Pub Co

In situ PCR is now a well-recognized method of detecting minute quantities of DNA or RNA in intact cells or tissue sections, providing a very valuable tool for answering many questions that could not have been otherwise investigated. A number of applications of this technology have been recently reported, bringing significant advancement to our understanding of many normal and pathological conditions, particularly in viral infections, tumor diagnosis, AIDS research and gene therapy. Despite its tremendous potential, most of its applications have not been fully explored. This book gives a timely, comprehensive review of the current status of this recent technology along with discussions of theoretical considerations. - Chapters written by some of the pioneers and foremost investigators of the in situ PCR technique. - Includes many step-by-step protocols. - Emphasis on real applications, especially molecular diagnostics.

In Situ Polymerase Chain Reaction and Related Technology

Science & Math

Gu, Jiang

Biological Sciences

Apoptosis Detection and Assay Methods (BioTechniques Molecular Laboratory Methods Series, 2)

Medical Univ. of South Carolina, Charleston. Presents protein staining procedures, including wool-dye, metallic, enzyme, flourescence, and immunostaining. Techniques for obtaining photographic or electronic images of gel separations are included. Outline. Plastic-comb binding.

Protein Staining and Identification Techniques (Biotechniques Molecular Laboratory Methods)

Allen, R.C.

Budowle, Bruce

Considers questions surrounding the origins of the AIDS virus, its relationship to simian immunodeficiency viruses, and chimpanzee immunity to HIV, and discusses intriguing new possibilities for the nature of human immunity and the development of an effective AIDS vaccine. Presents overwhelming evidence that neither humoral nor cell- mediated immunity can offer adequate protection against retroviral infections, and describes a new concept of natural defense that is operational against retroviruses. Ideas presented here will have an important impact on HIV vaccine development and on current lentiviral gene therapy protocols. Annotation c. Book News, Inc., Portland, OR (booknews.com)

HIV And Molecular Immunity: Prospects for the AIDS Vaccine

Medical Books

Bagasra, Omar

Basic Sciences

This major new two volume reference provides a comprehensive scientific and clinical review of the metabolic diseases, including clinical symptomatology, cardinal manifestations, molecular genetics, pathology and laboratory diagnostic features. There are thorough chapters on the major categories of disorders of metabolism as well as on various diagnostic approaches, prenatal diagnosis, and gene therapy. Pertinent information is presented in tables that highlight the text and by many illustrations liberally used throughout. Most tables include the six-digit numbers used in McKusick's Catalog of Genetic Diseases: Mendelian Inheritance in Man. 
The emphasis throughout this text is on clinical and pathologic expression of disease. The authors' primary aim, in conforming with the Osler tradition, is to present observed clinical phenomena along with the underlying physiologic and pathologic changes with regard to pertinent new discoveries in metabolism, including developments in molecular genetics. 
This text presents important new information and should be a ready, useful reference source to the pediatrician, internist, pathologist, clinical geneticist, and other physicians and scientists interested in metabolic diseases.

Metabolic Diseases: Foundations of Clinical Management, Genetics and Pathology (2-Volume Set)

Gilbert-Barness, Enid

Barness, Lewis A.

Barness, Lewis

Evolution

Contains papers from a September 1997 symposium, covering major subjects of concern to epidemiologists, pathologists, surgeons, oncologists, and molecular biologists. Papers are arranged in sections on epidemiology and pathology of pancreatic cancer, molecular biology of pancreatic cancer, and current and future treatment. Specific topics include cell linkage in pancreatic neoplasms, growth factors and their receptors in pancreatic neoplasia, and advances in surgery and imaging. Includes b&w and color photos. Annotation c. by Book News, Inc., Portland, Or.

Pancreatic Cancer: Advances in Molecular Pathology, Diagnosis & Clinical Management

Medicine

Tulane Medical School, New Orleans, LA. Provides a comprehensive review of recent developments in cancer genetics and biology. For researchers, clinicians, and students. Five sections discuss the genetic basis of cancer, while one section deals with the role of methylation and imprinting in cancer. DNLM: Neoplasms--genetics.

DNA Alterations in Cancer: Genetic and Epigenetic Changes

This book evolved from an earlier work of Weiner (454 Corporation) and Lu (GlaxoSmithKline), Cloning and Expression Vectors for Gene Function Analysis (2001) and has been designed to fill a perceived need for a text on technologies that addresses scaling up for high throughput, issues of modern cloning, and relatively unused techniques for protein expression and functional studies. They present 37 studies, organized into sections on bioinformatics, gene cloning technologies, gene expression technologies, in vitro gene expression technologies, protein purification technologies, and gene function analysis. Annotation c. Book News, Inc., Portland, OR (booknews.com)

Gene Cloning and Expression Technologies

Reprints 43 articles from the journal BioTechnique in sections on cloning vectors and strategies, protein expression and purification, gene tagging and epitope tagging strategies, reporter gene technologies, special purpose vectors, and in-vitro protein expression technologies. Among specific topics high-throughput transformation and plating using petristrips, the in-vivo labeling of over-expressed recombinant proteins in E. coli , the multiple epitope tagging of expressed proteins for enhanced detection, applications of green fluorescent proteins in plants, highly efficient eukaryotic gene expression vectors for peptide secretion, and the cell-free synthesis and affinity isolation of proteins on a nanomole scale. Updates have been added to some of the articles. Annotation c. Book News, Inc., Portland, OR (booknews.com)

Cloning and Expression Vectors for Gene Function Analysis (Biofocus (Westborough, Mass.).)

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