In Situ Polymerase Chain Reaction and Related Technology

In situ PCR is now a well-recognized method of detecting minute quantities of DNA or RNA in intact cells or tissue sections, providing a very valuable tool for answering many questions that could not have been otherwise investigated. A number of applications of this technology have been recently reported, bringing significant advancement to our understanding of many normal and pathological conditions, particularly in viral infections, tumor diagnosis, AIDS research and gene therapy. Despite its tremendous potential, most of its applications have not been fully explored. This book gives a timely, comprehensive review of the current status of this recent technology along with discussions of theoretical considerations. - Chapters written by some of the pioneers and foremost investigators of the in situ PCR technique. - Includes many step-by-step protocols. - Emphasis on real applications, especially molecular diagnostics.