Methods in Neurotransmitter Receptor Analysis

Every neuroscientist and neuropharmacologist will welcome this how-to laboratory guide to neurotransmitter receptor binding assays. This manual details recent refinements in assay procedures and data analysis and describes a variety of methods, including procedures for studying receptor-mediated second messenger production and accumulation. Other topics covered include use of cultured cells for defining receptor systems and in situ hybridization for producing these membrane components. The book provides the practical information needed to establish these assays in the laboratory, including lists of supplies and step-by-step protocols. Helpful examples show how the procedures are performed. The authors also explain the strengths, weaknesses, and applications of each assay and note common difficulties in performance and/or data analysis.