MEMBRANE ANALYSIS (Introduction to Biotechniques (BIOS))

This volume provides an introduction to the basic principles and the m ethods used to prepare biological membranes for analysis of their mole cular composition and structure. The book also covers the methodology associated with the dissection of membrane trafficking. In addition, t he choice of biological material and technique is often crucial and Me mbrane Analysis provides expert guidance on the most appropriate metho d for the job. Topics covered include the source of membranes, prepara tion of subcellular membranes, molecular composition, structural organ ization of membrane proteins and glycoproteins, structural organizatio n of membrane lipids and glycolipids, membrane assembly and protein ta rgeting, membrane transit and secretion, and receptor-mediated endocyt osis and membrane trafficking.