In Vitro Culture of Higher Plants presents an up-to-date and wide- ranging account of the techniques and applications, and has primarily been written in response to practical problems. Special attention has been paid to the educational aspects. Typical methodological aspects are given in the first part: laboratory set-up, composition and preparation of media, sterilization of media and plant material, isolation and (sub)culture, mechanization, the influence of plant and environmental factors on growth and development, the transfer from test-tube to soil, aids to study. The question of why in vitro culture is practised is covered in the second part: embryo culture, germination of orchid seeds, mericloning of orchids, production of disease-free plants, vegetative propagation, somaclonal variation, test-tube fertilization, haploids, genetic manipulation, other applications in phytopathology and plant breeding, secondary metabolites.

In Vitro Culture of Higher Plants

Science & Math

Pierik, R.L.M

Biological Sciences

Springer

CULTIVO IN VITRO DE LAS PLANTAS SUPERIORES

Reference

Foreign Language Study & Reference

Ediciones Mundi-Prensa

16. 2. Techniques 141 16. 3. Factors affecting the success of embryo culture 143 16. 4. Practical applications . . 146 17. Germination of orchid seeds . . . . . . . 149 17. 1. Introduction . . . . . . . . . . . . . 149 17. 2. Factors affecting germination and growth 154 18. Vegetative propagation of orchids 159 18. 1. Introduction . . . . . . . . . 159 18. 2. Meristem culture . . . . . . . 161 18. 2. 1. Production of virus-free plants 161 18. 2. 2. Propagation through meristem culture 162 18. 3. Other methods of propagation 165 18. 4. Variation arising during culture 166 18. 5. Practical applications . . . . 167 19. Production of disease-free plants 169 19. 1. Introduction . . . . . . . . 169 19. 2. Production of virus-free plants 171 19. 2. 1. Heat treatment . 171 19. 2. 2. Meristem culture . 172 19. 2. 2. 1. History . . . . 172 19. 2. 2. 2. Accomplishment 174 19. 2. 3. Heat treatment and meristem culture 177 19. 2. 4. Adventitious shoot formation, eventually followed by - ristem culture . . . . . . . . . . . . . . . . . . 177 19. 2. 5. Virus-free plants produced from callus and protoplasts. 178 19. 2. 6. Grafting of meristems on virus-free (seedling) rootstocks (micrografting) . . . . . . . . . . . . . . . . . . 179 19. 2. 7. Virus identification . . . . . . . . . . . . . . . . 179 19. 3. Production of bacteria- and fungal-free plants by meristem culture . . . . . . 180 20. Vegetative propagation 183 20. 1. General introduction 183 20. 2. Single-node culture . 190 20. 3. The axillary bud method 194 20. 4. Regeneration of explants 200 20. 4. 1. Introduction . . . . . 200 20. 4. 2. Adventitious root formation 203 20. 4. 3. Adventitious shoot formation 209 III 20. 5.

Pierik, R.L.

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