In Situ Hybridization in Electron Microscopy (Methods in Visualization)

Science & Math

Williams, Lynda

Morel, Gérard

Cavalier, Annie

Biological Sciences

CRC-Press

Although the polymerase chain reaction has revolutionized genetic analysis by amplifying rare nucleic acid sequences, the in situ application is the only method that allows the localization of amplified signal within tissue structure. The applications of in situ polymerase chain reaction have greatly enhanced the field of investigation in many disciplines, including viral infections, gene modification, tumor diagnosis, gene therapy, and cellular distribution of rare mRNA copies. PCR/RT-PCR in situ: Light and Electron Microscopy covers methods of in situ polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR), two new approaches in visualizing very low amounts of DNA and RNA in tissues and cell cultures at the light and electron microscopy levels. Written by experts in this field, the book provides theoretical consideration, as well as practical approaches to in situ PCR. The authors provide detailed protocols for each step, including the preparation of tissue samples, the rationale for the design of primers and revelation. They also emphasize the need for appropriate controls to meet the requirements of in situ PCR and RT-PCR specificity. Organized in a user-friendly two-column format, this book will provide you with tools necessary to perform and optimize these sensitive and powerful techniques in your research protocols.

PCR/RT- PCR in situ (Methods in Visualization)

Raccurt, Mireille

In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells, and is an especially important method for studying nucleic acids in heterogeneous cell populations. in situ Hybridization in Electron Microscopy reviews the three main methods developed for the ultrastructural visualization of genes: ° hybridization on ultrathin sections of tissue embedded in hydrophilic resin (post-embedding method) ° hybridization prior to embedding (pre-embedding) ° hybridization on ultrathin sections of frozen tissue (frozen tissue method). For each technique, the different stages are described in detail: the preparation of tissue, pretreatment, hybridization, and visualization of the hybridization products. The book combines theory and practice, starting with the basic principles, then breaking down the experimental process into successive steps illustrated by numerous diagrams, detailed protocols, and tables. This is all done in a format that uses parallel columns to convey useful comments next to the theory and practical details alongside each stage of the protocol. Additionally, the summary tables provide the criteria for choosing the probe type and technique, and a detailed index aids in the search for information. in situ Hybridization In Electron Microscopy is an essential companion for applying these methods at the electron microscopic level.

In Situ hybridization allows the visualization of specific DNA/RNA sequences in individual cells in tissue sections, single cells, or chromosome preparations, and is an especially important method for studying DNA and RNA in heterogeneous cell populations. This book delves into in situ hybridization methods through the use of light microscopy used by molecular biologists, pathologists, geneticists, and biochemists. It will also appeal to research scientists who are interested in visualizing methods for nucleic acids and proteins. The book features a two-column layout for protocols/principles, simplifies the techniques and offers many schemes and tables to help the reader choose the best procedure.

In Situ Hybridization in Light Microscopy (Methods in Visualization)

A multiplicity of methods for visualization are described in Visualization of Receptors: Methods in Light and Electron Microscopy. This book of techniques covers immunocytology, radioautography, in situ hybridization, plasmon resonance, RT in vitro PCR, and X-ray crystal structure analysis. Lecturers, researchers, practitioners, technicians, and students will find all the principles and protocols they require--and the means of implementing them. Topics discussed include: localization of ligands (in vitro or in vivo); visualization of immunoreactivities of ligand and receptor proteins; detection and quantification of mRNA expression; amplification of signals; determination of 3-D structure. Details of protocols with illustrations of results and commentaries are provided throughout the book.

Visualization of Receptors: Methods in Light and Electron Microscopy

Madame Veuve

YSEC

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Books by Morel, Gérard

See the best price to sell, buy, or rent books by Morel, Gérard. BookScouter helps to compare book prices from 25+ online bookstores and 30+ buyback vendors with a single search.

In Situ Hybridization in Electron Microscopy (Methods in Visualization)

PCR/RT- PCR in situ (Methods in Visualization)

In Situ Hybridization in Electron Microscopy (Methods in Visualization)

In Situ Hybridization in Light Microscopy (Methods in Visualization)

Visualization of Receptors: Methods in Light and Electron Microscopy

Madame Veuve