<p>This book has a distinguishing feature of having condensed material with adequate information on genetic engineering especially of the microbes. The book covers almost all the topics of genetic engineering for the graduate, postgraduate students and young research scholars of biological sciences. The book is written as per syllabus of genetic engineering paper for Masters course in biotechnology, biochemistry, life sciences of most of the universities. The book is much useful for the students of Masters degree. Emphasis is given on the basic fundamentals. The book contains twelve chapters starting from ' Isolation, purification and estimation of nucleic acids' as chapter 1. The chapter describes general techniques for the isolation and purification of DNA as well as RNA. It also describes methods for quantitative estimation of the nucleic acids. The second chapter describes general characteristics of the vectors used in genetic engineering and also the general account of commonly used individual vectors. The chapter also describes expression vectors. The third chapter describes various commonly used restriction endonucleases. viz. Reverse transcriptase, DNA polymerase I, polynucleotide kinase, teminal dcoxynucleotidyl transferase, alkaline phosphatase, SI nuclease, DNA ligase etc. The fifth chapter describes electrophoresis for the separation of nucleic acids fragments. The sixth chapter is of cloning strategies. It describes construction of genomic DNA library, chromosomal walking, cDNA library, cDNA cloning. The seventh chapter describes DNA sequencing techniques and includes chemical modification method of Maxam and Gilbert, dideoxy sequencing method of Sanger, modifications of chain terminator sequencing, analysis of the sequencing data. The eighth chapter includes various methods of site directed mutagenesis. The ninth chapter describes polymerase chain reaction (PCR). It also includes primer designing and various types of polymerase chain reactions viz. reverse transcriptase polymerase chain reaction (RT-PCR), nested PCR, multiplex PCR etc. Besides, there are chapters 10, 11 and 12 on gene therapy, human genome and proteomics. At the end, glossary has been put which explains main terms used in genetic engineering. given in the beginning of each chapter that provides, at a glance, the contents of the whole chapter which offers a better learning mechanism. Each chapter is also presented with an introduction that covers the concept of the whole chapter in brief and offers clear understanding of the subject matter to the students. The author on the basis of his experience in teaching genetic engineering at the university level for more than a decade has offered the text in an easily understandable form to the postgraduate students. The book should be of invaluable help to the students, researchers and all those interested in understanding genetic engineering.</p>

Genetic Engineering

Science & Math

Garg, Neha

Kumar, Anil.

Biological Sciences

Nova Science Pub Inc

The book will be useful to master's, bachelor's and doctoral students in biological sciences. It covers most of the practicals conducted at the master's level in biological sciences. The book contains 20 chapters describing practical protocols. The book starts from the chapters 'Colorimetry' and 'Spectrophotometry' where theoretical parts of these techniques are described. The book has a chapter that describes all the techniques used in plant tissue culture. The most important and salient feature of the book is that principles of the practical exercises as well as detailed protocols are described. Besides, the book covers principles and theoretical parts of many modern techniques, namely chromatography, electrophoresis, UV-VIS spectrophotometry, centrifugation, PCR and plant tissue culture.

Biochemical Tests: Principles and Protocols

Politics & Social Sciences

Garg, Sarika

Social Sciences

MV Learning

<p>The book has been written in such a way that the concepts and working of algorithms are explained in detail, with adequate examples. To make clarity on the topic, diagrams, calculation of complexity, algorithms are given extensively throughout. Many examples are provided which are helpful in understanding the algorithms by various strategies. This content is user-focused and has been highly updated including algorithms and their real-world examples. Key features This book is especially designed for beginners, and explains all aspects of algorithm and its analysis in a simple and systematic manner. Algorithms and their working are explained in detail with the help of several illustrative examples. Important features like greedy algorithm, dynamic algorithm, string matching algorithm, branch and bound algorithm, NP hard and NP complete problems are suitably highlighted. Solved and frequently asked questions in the various competitive examinations, sample papers of the past examinations are provided which will serve as a useful reference source. The book would serve as an extremely useful text for BCA, MCA, M. Sc. (Computer Science), PGDCA, BE (Information Technology) and B. Tech. and M. Tech. students. Contents Algorithm & Algorithmic Strategy Complexity of Algorithms Divide-and-Conquer Algorithms Greedy Algorithm Dynamic Programming Graph Theory Backtracking Algorithms Branch and Bound Algorithms String-Matching Algorithms P and NP Problems</p>

Analysis and Design of Algorithms: A Beginner's Hope

Computers & Technology

Singhal, Shefali

Programming

BPB Publications

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