<p>PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.</p>

PCR Cloning Protocols (Methods in Molecular Biology)

Science & Math

Bing-Yuan Chen

Biological Sciences

Humana Press

See the best price to sell, buy, or rent books by Bing-Yuan Chen. BookScouter helps to compare book prices from 25+ online bookstores and 30+ buyback vendors with a single search.

Books by Bing-Yuan Chen

See the best price to sell, buy, or rent books by Bing-Yuan Chen. BookScouter helps to compare book prices from 25+ online bookstores and 30+ buyback vendors with a single search.

PCR Cloning Protocols (Methods in Molecular Biology)