Gene Targeting and Embryonic Stem Cells (Advanced Methods)

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Gene Targeting and Embryonic Stem Cells (Advanced Methods) image
ISBN-10:

1859963609

ISBN-13:

9781859963609

Edition: 1
Released: Jul 01, 2004
Publisher: Taylor&Francis
Format: Paperback, 216 pages

Description:

Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field.

Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference.

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