Manipulation and Expression of Recombinant DNA: A Laboratory Manual

This laboratory manual will be an indispensable tool for students, by providing in depth descriptions of protocols which cover the basic aspects of molecular biology and biochemistry. The book covers techniques in detail, and teaches students to culture and transform bacteria, grow vector plasmids, purify insert DNA, ligate vector and insert, and ultimately express protein and assay its activity. Comprehensive and complete, this text covers the underlying techniques used in all basic research and biotechnology laboratories. Key Features* Designed to cover the basic aspects of molecular biology and biochemistry* Features student-tested protocols* Presents experiments in a modular fashion, lending itself equally to the traditional semester course and the shorter intensive course.* Covers important techniques and protocols used in all basic research labs* "Project" approach to experiments gives students an overview of entire process* Experiments incorporate immunological techniques that go beyond basic exercises in DNA cloning