In the genome era, the analysis of gene expression has become a critical requirement in many laboratories. But there has been no comprehensive source of strategic, conceptual, and technical information to guide this often complex task. Transcriptional Regulation in Eukaryotes answers that need. Written by two experienced investigators, Michael Carey and Stephen Smale at the UCLA School of Medicine, and based in part on the Gene Expression course taught at Cold Spring Harbor Laboratory, this book directly addresses all the concerns of a laboratory studying the regulation of a newly isolated gene or the biochemistry of a new transcription factor. This book is essential reading for anyone pursuing the analysis of gene expression in model systems or disease states.

Transcriptional Regulation in Eukaryotes: Concepts, Strategies and Techniques

Science & Math

Carey, Michael

Smale, Stephen T.

Biological Sciences

Cold Spring Harbor Laboratory Pr

In the genome era, the analysis of gene expression has become a critical requirement in many laboratories. But there has been no comprehensive source of strategic, conceptual, and technical information to guide this often complex task. "Transcriptional Regulation in Eukaryotes" aims to answer that need. Written by two experienced investigators, Michael Carey and Stephen Smale at the UCLA School of Medicine, and based in part on the Gene Expression course taught at Cold Spring Harbor Laboratory, this book directly addresses all the concerns of a laboratory studying the regulation of a newly isolated gene and the biochemistry of a new transcription factor. Chapter 1 reviews the state-of-the-art in the RNA polymerase II transcription field; while chapters 2 to 10 are a systematic guide to the investigation of regulatory mechanisms for newly identified genes. Chapters 11 to 15 discuss approaches for synthesizing recombinant transcription factors, analyzing their binding, and deciphering th

Transcriptional Regulation in Eukaryotes: Concepts, Strategies, and Techniques

The first edition of the highly successful Transcriptional Regulation in Eukaryotes, written by Michael Carey and Stephen Smale at UCLA, provided a comprehensive source of strategic, conceptual, and technical information for investigating the complexities of gene regulation at the level of transcription. With the ever-increasing importance of genome data and the appearance of new and better techniques, the second edition of this book has added a third author, Craig Peterson at the University of Massachusetts Medical School. In addition to a new chapter on the in vitro analysis of chromatin templates for DNA-binding studies and transcription, this second edition has been extensively rewritten and updated to discuss new advances in the field and their impact on gene regulation mechanisms. The second edition retains the approach of the first in covering both the conceptual and practical aspects of how to study the regulation of a newly isolated gene and the biochemistry of a new transcription factor. Transcriptional Regulation in Eukaryotes serves as both a powerful textbook and manual for advanced instruction in molecular biology which -supplements clearly written text with extensive illustrations -puts methods in the context of underlying theory -gives expert recommendations on experimental strategies -encourages creativity in investigative design -explains protocols for essential techniques step by step, with extensive advice on troubleshooting -provides the latest methods in use in the field This important and unique book is essential reading for anyone pursuing the analysis of gene expression in model systems or disease states, providing underlying theory and perspective to the newcomer and the latest techniques to the expert.

Carey, Michael F

Peterson, Craig L

Cold Spring Harbor Laboratory Press

Strategies for studying gene regulation mechanisms have changed dramatically over the past several years in light of the emergence of complete genome sequences for many organisms as well as the development of or improvements to technologies such as chromatin immunoprecipitation, RNA interference, microarrays, and proteomics. The first edition of the highly successful Transcriptional Regulation in Eukaryotes, written by Michael Carey and Stephen Smale at UCLA, provided a comprehensive source of strategic, conceptual, and technical information for investigating the complexities of gene regulation at the level of transcription. With the ever-increasing importance of genome data and the appearance of new and better techniques, the second edition of this book has added a third author, Craig Peterson at the University of Massachusetts Medical School. In addition to a new chapter on the in vitro analysis of chromatin templates for DNA-binding studies and transcription, this second edition has been extensively rewritten and updated to discuss new advances in the field and their impact on gene regulation mechanisms. The second edition retains the approach of the first in covering both the conceptual and practical aspects of how to study the regulation of a newly isolated gene and the biochemistry of a new transcription factor. Transcriptional Regulation in Eukaryotes serves as both a powerful textbook and manual for advanced instruction in molecular biology which -supplements clearly written text with extensive illustrations -puts methods in the context of underlying theory -gives expert recommendations on experimental strategies -encourages creativity in investigative design -explains protocols for essential techniques step by step, with extensive advice on troubleshooting -provides the latest methods in use in the field This important and unique book is essential reading for anyone pursuing the analysis of gene expression in model systems or disease states, providing underlying theory and perspective to the newcomer and the latest techniques to the expert.

Transcriptional Regulation in Eukaryotes: Concepts, Strategies, and Techniques, Second Edition

See the best price to sell, buy, or rent books by Smale, Stephen T.. BookScouter helps to compare book prices from 25+ online bookstores and 30+ buyback vendors with a single search.

Books by Smale, Stephen T.

See the best price to sell, buy, or rent books by Smale, Stephen T.. BookScouter helps to compare book prices from 25+ online bookstores and 30+ buyback vendors with a single search.

Transcriptional Regulation in Eukaryotes: Concepts, Strategies and Techniques

Transcriptional Regulation in Eukaryotes: Concepts, Strategies, and Techniques

Transcriptional Regulation in Eukaryotes: Concepts, Strategies, and Techniques

Transcriptional Regulation in Eukaryotes: Concepts, Strategies, and Techniques, Second Edition