Manual includes five sections: overview of some key aspects of phage display; the construction, screening, and analysis of antibody libraries on the surface of phage, of phage-displayed peptide libraries, and of gene-fragment and cDNA-expression libraries; and appendices that summarize commonly used experimental procedures, data, recipes, suppliers, and precautions.

Phage Display: A Laboratory Manual

Science & Math

Burton, Dennis R.

Silverman, Gregg J

Scott, Jamie K.

Biological Sciences

Cold Spring Harbor Laboratory Pr

Phage-display technology has begun to make critical contributions to the study of molecular recognition. DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA. Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage is amplified by infection of bacteria. Phage-display technology is powerful but challenging and the aim of this manual is to provide comprehensive instruction in its theoretical and applied so that any scientist with even modest molecular biology experience can effectively employ it. The manual reflects nearly a decade of experience with students of greatly varying technical expertise andexperience who attended a course on the technology at Cold Spring Harbor Laboratory. Phage-display technology is growing in importance and power. This manual is an unrivalled source of expertise in its execution and application.

Barbas III, Carlos F

Cold Spring Harbor Laboratory Press

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